Peasy t1

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WebpEASY® -T1 Cloning Kit 目录号： CT101-01 单价：￥620 规格： 20 rxns 60 rxns 数量： - + 说明书载体信息质检报告立即下单加入购物车产品详情介绍 pEASY®-T1 Cloning … WebpEASY® -T1 Cloning Kit Catalog Number：CT101-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now Add to …

pEASY® -T3 Cloning Kit - TransGen Biotech

WebAug 1, 2024 · A Positive positive reference plasmid for Brucella spp. detection (pEASY-T1- omp2a) was constructed by our lab. TwistAmp exo and TwistAmp basic Kits were provided by TwistDx (Cambridge, UK). DNase/RNase-Free distilled, deionized water (DDH2O) was provided by Tiangen Biochemical Co., Ltd. WebApr 6, 2024 · All PCR products were separated on a 1.2% agarose gel, and the target bands were purified and then ligated into the pEASY-T1 vector (TransGen, Beijing, China). The PCR products were sequenced by Sangon Biotech (Shanghai, China) [ 25 ]. minecraft videos siren head

pEASY® -T1 Simple Cloning Kit - TransGen Biotech

WebApr 1, 2024 · pEASY-T1 vector. Restriction Map. Enzyme # of cuts positions Acc65I (1) ggtacc 246 AceIII (3) cagctc 948, 2629, 3869 AciI (47) ccgc 88, 191, 328, 560, 574, 1013, 1158, 1514, 1578, 1679 AclI (2) aacgtt 2318, 2691 AcuI (4) ctgaag 3282, 1575, 2007, 2234 AflIII (2) acrygt 1020, ... WebPeasy T1 Simple Cloning Vector System, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebOct 15, 2024 · To compare the detection sensitivities between dLAMP and qPCR, we gradient diluted pEASY-T1-mpb70 plasmid and added them to dLAMP and qPCR systems, respectively. The qPCR detection resulted in good linearity between input templates and measured concentration (R 2 = 0.9977; Fig. 3); a wide range of templates can be … mortola library hours

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pEASY®-Blunt E1 Expression Kit - TransGen Biotech

Webpeasy 系列克隆载体; 相关产品; 克隆感受态细胞; 点突变; 核酸纯化 . 基因组dna纯化; 质粒dna纯化; dna产物纯化; rna纯化; 相关产品; 二代测序 . dna建库; rna建库; 相关产品; mngs; … WebpEASY-T1 Simple (linearized) TA cloning vector suitable for blue-white selection, with kanamycin and ampicillin resistance markers and a T7 promoter but no multiple cloning … mortoen bay libraryWebpEASY ® - T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 … morto fred ward

"WebThis fragment contains 285 bp upstream of the Hsp-3start codon and was ligated into the T1 vector using the pEASY-T1 Cloning kit (Transgen) (Figure 1, step 2) to generate sufficient material for subsequent manipu- lations. " - Peasy t1

Peasy t1

Overexpression of the cotton trihelix transcription factor

WebMar 15, 2024 · Purified PCR products were cloned and constructed pEASY-T1-CGMMV, pEASY-T1- A. citrulli, and pEASY-T1- EF-1α (540-bp) plasmid by using a pEASY-T1 Simple Cloning Kit (TransGen Biotech, Beijing, China), respectively. WebpEASY -T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products (3′-A overhangs). The vector is suitable with T7 RNA polymerase-mediated in vitro RNA …

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WebMar 2, 2024 · The amplified GhGT23 fragment was subcloned into pEASY-T1 (TransGen Biotech, Beijing, China), thereby creating pEASY-T1::GhGT23. Flanking BamHI and SalI restriction sites were then used to cut GhGT23 from pEASY-T1::GhGT23, followed by ligation into the pBI21, pBin438, pGAL4 DBD, and pGEX6p-1 expression vectors. WebBraccialini - Braccialini - Accessoires textile - Accessoires sacs - Parapluies - Rose 60,00 € - Spartoo

WebM-Wave Drag-T1 M-Wave Drag-T2 M-Wave Peasy; Zum Angebot * x Wir verlinken auf ausgewählte Online-Shops und Partner, von denen wir ggf. eine Vergütung erhalten. Zwischenzeitliche Änderung der Preise, Lieferzeit und -kosten möglich. Preise inkl. MwSt, ggf. zzgl. Versand. Wenn Sie eine ältere Version des Vergleichs aufrufen oder ansehen ... WebContext 1 ... determine the detection limit, PCR and LAMP were performed with tenfold serial dilutions of the plasmid pEASY ® -T1-B637 as template. PCR products were detected by agarose gel elec-...

WebMar 30, 2024 · Escherichia colicloning vector pEASY-T1, expression vector pEASY-E2, competent cells Trans1-T1 and Transetta (DE3) were purchased from TransGen Biotech (Beijing, China). The whole gene of the mature form of ANGPTL8/betatrophin was synthesized by Inovogen Tech. Co. (Beijing, China) into pUCE plasmids. WebpEASY ® - T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. ... •Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage Competent Cell at -70°C for six months; others at -20 ...

WebJan 30, 2015 · The amplified DNA was purified using an AxyPrep DNA gel extraction kit (Axygen), inserted into the pEASY -T1 Cloning Vector (TransGen, Beijing, China) and transformed into E. coli Trans 1-T1 competent cells (TransGen, Beijing, China). Positive clones were analyzed by PCR using vector-specific primers.

WebApr 24, 2015 · The reference plasmid pEASY-T1-STY2879 was constructed as follows: the full length STY2879 gene, which was from the S. Typhi strain CT18, was amplified by a set of primers, STY2879-F: 5’-3’ ttacaacttaaagagtttcgc and STY2879-R: 5’-3’ atgacaatagaagttcaaagg, and the PCR products (774 bp) were ligated into the pEASY-T1 … mor toiletries bagWebpEASY-T3 (linearized) TA cloning vector that allows the cloned PCR product to be excised with EcoRI or NotI. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets No matches minecraft videos with aft mouthWebpEASY® – T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®- T1 Cloning vector. It is designed for cloning and sequencing Taq -amplified PCR products. … minecraft videos with gunsWebpEASY -Blunt Cloning Kit 使用前请务必仔细阅读说明书目录号：CB101 保存:Trans1-T1 Phage Resistant Chemically Competent Cell-70℃保存六个月，其它-20℃保存六个月。 *片段为胶回收产物，反应时间取最大值。 5、最佳反应温度：25℃ *若片段是高GC结构，可以37℃反应。（推荐用PCR 仪控温） ® 试剂盒组成 pEASY- Blunt Cloning Vector (10 … mor toiletry bagsWebpEASY T1 载体 _生物学_自然科学_专业资料。 pEASYpEASY ?-T1 Cloning Kit 目录号:CT...测序用 M13 F,M13 R,T7 promoter 引物测序,进行序列分析。注意! 1. 根据... pEASY -Blunt clonning vector 载体说明书WORD版 pEASY -Blunt clonning vector 载体说明书WORD版_生物学_自然科学_专业资料。 pEASY ...bases 3134-3807 2 pEASY -Blunt Restriction Map … morton and associates food brokersWeb1.1.4 主要试剂 Mini BEST Viral RNA/DNA Extraction Kit Ver.5.0，购自TaKaRa公司；琼脂糖凝胶DNA回收试剂盒、快速质粒小提试剂盒，购自天根生物科技有限公司；PEASY-T1 Simple Cloning Kit，购自北京全式金生物技术有限公司；TaqPCR Master Mix (2×)，购自生工生物工程(上海)股份有限 ... minecraft videos that you can watchpEASY-T1 (linearized) TA cloning vector suitable for blue-white selection, with kanamycin and ampicillin resistance markers and a T7 promoter. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets No matches mor toledano\u0027s daughter leeya eliana shapiro