WebSep 1, 2024 · One issue that can arise with the optopatcher is unwanted light leak due to the transparency of the pipette, illuminating a wide area around the walls of the pipette as well as the tip itself (the diameter of the beam at the tip of the pipette is about 800 μm). WebNov 8, 2013 · The optopatcher is a new micropipette holder with integrated optical fiber to allow simultaneous patch-clamp recording and optogenetic activation. The design eliminates the need for a separate manipulator for optical stimulation. The optopatcher was developed by A-M...
Optopatcher–An electrode holder for simultaneous …
WebMay 19, 2024 · We used the optopatcher for online identification of direct and indirect pathway projection neurons (MSNs) during in vivo whole-cell recordings. In control mice, MSNs encoded the laterality of sensory inputs with larger and earlier responses to contralateral than ipsilateral whisker deflection. WebNov 8, 2013 · The optopatcher is a new micropipette holder with integrated optical fiber to allow simultaneous patch-clamp recording and optogenetic activation. The design eliminates the need for a separate manipulator for optical stimulation. china polyester fleece jacket
Optopatcher—An electrode holder for simultaneous
WebHere we used the optopatcher in vivo to record the membrane potential of neurons from different cortical layers in the motor cortex of transgenic mice expressing channelrhodopsin-2 under the Thy1 promoter. We demonstrate the utility of the optopatcher by recording LFP and intracellular responses to light stimulation. WebNov 8, 2013 · The optopatcher is a new micropipette holder with integrated optical fiber to allow simultaneous patch-clamp recording and optogenetic activation. The design … WebIllustration shows optopatcher recordings containing an optic fiber and silver-chloride wire pho-tostimulating the recorded MSN (right). Responses to light in D2-ChR2-YFP mouse (ChR2+, top) appeared as a depolarizing step for the duration of the stimulation superimposed by APs. Inset shows magnification of the light response onset. gram free online