Nettet20. des. 2024 · M. Bohndorf, A. Ncube, L.-S. Spitzhorn, J. Enczmann, W. Wruck, and J. Adjaye, “Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 4/ 17 variant which confers intermediate drug metabolizing activity,” Stem Cell … Nettet10. jul. 2024 · Therefore, integration-free methods for iPSC derivation are desired for manufacturing clinical-grade cells. Several non-integrating methods, including recombinant proteins, DNA plasmids, and Sendai virus, have been developed to transiently express transcription factors to induce reprogramming (Okita et al., 2011 ; Seki et al., …
Supporting dataset of two integration-free induced pluripotent stem ...
Nettet18. okt. 2012 · Our data indicate that this episome-mediated reprogramming is not inherently mutagenic during integration-free iPSC induction. More recently, we replaced mouse feeder cells with engineered human ... Nettet21. mai 2013 · We have successfully generated integration-free iPSCs from cord blood (CB) CD34 + cells with improved oriP/EBNA1-based episomal vectors (EV) using a strong spleen focus forming virus (SFFV) long terminal repeat (LTR) promoter. mercusys mw300uh n300
Generation of an integration-free iPSC line (CSCRi005-A) from …
Nettet21. mai 2013 · Generation of integration-free iPSCs with episomal vectors. PBMNCs or fractionated cells (CD33 + or CD3 − /CD19 −) cells) were cultured under HSC … Nettet7. jul. 2015 · ( a) Integration-free iPSC generation method. ( b) Morphology and alkaline phosphatase (AP) staining of integration-free iPSCs. Scale bars, 100 μm. ( c) PCR analysis using seven primers... NettetReprogramming of somatic cells with higher genome integrity, and use of non-integrating gene delivery methods and xeno-free cell culture conditions aid in the generation of … mercusys mw300re 300mbps wi-fi range extender