WebMIGR1 constructs carrying the human Flt3, W51 and W81 Flt3-ITD coding sequences were kindly provided by Dr. Gary Gilliland (Harvard Institute of Medicine). The constitutively active mutant murine STAT5A(1 ∗ 6) was subcloned from pMXpuro-STAT5(1 ∗ 6) into the EcoRI-SalI sites of the pIRES2-EGFP vector (Clontech, Palo Alto, CA, USA). WebJan 1, 2002 · FLT3-ITD mutants were cloned from primary human leukemia samples and assayed for transformation of primary hematopoietic cells using a murine bone marrow …
Differential Inhibition of FLT3-ITD, FLT3-WT, and KIT By
WebAug 22, 2005 · vav-FLT3-ITD transgenic construct. Human FLT3 cDNA harboring a W51 ITD mutation (Kelly et al., 2002) was cloned into the HS321/45 vav hematopoietic vector … WebJun 27, 2016 · The empty vector, MSCV-IRES-eGFP-FLT3-WT and MSCV-IRES-eGFP-FLT3-W51 have been described previously 11. For the stable transduction of Ba/F3 cells the retroviral supernatant of Phoenix eco cells ... ea play bf2024
Recurring chromosomal abnormalities in leukemia in
WebFeb 1, 2005 · Enforced expression of wild-type Flt3-RTK and W51 Flt3-ITD in human CB-CD34 ؉ cells. (A) Map of MIGR1, wt Flt3 , and W51 Flt3-ITD retroviruses used in CA frequencies determined by limiting ... WebAug 1, 2003 · Activating FLT3 mutations have been identified in approximately 35β of APLs, most of which result from an internal tandem duplication (ITD) of the juxtamembrane domain. 15 All of the leukemias arising in the PML-RARA/FLT3 W51 mice had abnormal karyotypes; however, the karyotypes were very simple with 6 (86β) of 7 showing loss of … Web( C ) Down-regulation of FLT3 occurs in Ba/F3 W51 cells only below oxygen levels of 6% O 2 . ( D ) Ba/F3 W51 cells down-regulate FLT3 after 48 hours of culture under hypoxia. Fold change... csr in bpo