WebMar 6, 2024 · Loss of function mutations in the NPC1 gene lead to the build-up of cholesterol in the endo-lysosomal compartment and a progressive neurodegenerative disorder, NPC disease, which leads to premature death. 11 Genetic testing together with staining of patient cells with filipin, a fluorescent cholesterol-binding compound, are … WebDec 16, 2024 · Unlike filipin, where the scientific community accepts that it only reacts with unesterified cholesterol, FP-5 probe appears to be associated with both entities, i.e. cholesterol and cholesterol ...
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WebCatalog number: N1142. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes … WebHi Michal, There is a filipin (compound similar to digitoxin) method for free cholesterol. Fixation and sections: cryostat sections, post-fixed; frozen sections of fixed tissue. Stock … WebDescription. Designed for minimal autofluorescence interference, the Amplex Red Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluorometer. Helps accurately measure cholesterol content of 0.01μL of human serum. features of people management